Sentence examples for round of amplified from inspiring English sources

In some cases, the first round of amplified products were not detectable on the agarose gels (data were not shown), but target products could still be obtained in the next PCR.

In the first round of PCR amplified with primers CALL001 and CALL002, the resulting PCR fragments had two distinct bands at around 700 and 900 bp corresponding to VH-CH1-CH2 and VHH-CH2 genes, respectively (Fig. 3 lane 2).

Finally, the second round of PCR amplified matK pseudogenes in four species (Amerorchis rotundifolia, Limnorchis aquilonis, Limnorchis huronensis, Salix myrtillifolia - Additional file 2).

2nd round: 2 μl of the first round PCR was amplified with 25pmol of each inner primer in 50 μl using FastStart Taq polymerase (Roche) in the supplied reaction buffer containing 200 μm each dNTP and 1x GC-RICH solution (Roche).

At last, the diluted PCR products of the second round were amplified for another nested PCR with PPT primer and UP primer.

The DNA pool collected after each round of selection was amplified by PCR, and the product was used to prepare ssDNA for the next round of selection.

The eluted pool for each round of SELEX was amplified through PCR, after which the ssDNA pools of interest were recovered and monitored for enrichment toward TOV-21G by flow cytometry.

The cap gene region containing the oligonucleotide insert of AAV recovered from breast cancer cells after each round of selection was amplified by nested PCR and correct size of the amplification product was verified by agarose gel electrophoresis (data not shown).

All samples tested positive for B19V were reconfirmed with an independent second round of PCR which amplified the NS1/VP1u-region [ 31].

The second round of PCR was amplified with the F1 and R2 primers using the first PCR product as the template.

We next diluted each sample 100-fold and used the diluted samples in a second round of PCR which amplified the libraries and incorporated the appropriate Illumina sequencing adapters and barcodes.