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The old round mouse was a case study in the hazards of putting form over function.
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It replaces the round Apple mouse, which was derided by product reviewers as "an ergonomic nightmare" and a "hockey puck".
In each inoculation round knockout mice and an equal number of wild-type mice were inoculated with the same bacterial inoculum to control for variations between inocula.
The mouse-adapted variant that was re-sequenced in this study was the inoculum that was used to infect the final round of mice and had been passaged three times.
5 10 µl blood was collected in 1 ml Alseviers' solution from the tail vein of a second round-selection mouse, the blood was centrifuged at 2000 rpm for 5 min, the supernatant was aspirated off, and the pellet was suspended in 3 ml RPMI1640 (supplemented with 20% FBS), and transferred to a 50 ml sterile tube.
2R, two rounds; MMTV, mouse mammary tumor virus.
I have to confess, this was not our first go-round with mice.
After several rounds, the mice froze in fear at the sound.
C The second round, four WT mice and four ob/ob mice.
Mouse round spermatids the youngest haploid male germ cells appear first at 17 days after birth and can be used for the production of offspring by round spermatid injection (ROSI) into oocytes [9].
It is expressed in mouse round spermatids and mature oocytes and is known to interact with both DNA and RNA.
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