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Global alignments of the amino acid sequences of the κ-opioid receptor from sheep, zebrafish, rough skinned newt, mouse and guinea pig return similarity scores of 60 99%.
Six laboratory-housed rough skinned newts (Taricha granulosa), were sampled via tail snips with sterile surgical blade, 30 µl of blood was then collected from the caudal vein into microhematocrit capillary tubes and transferred to sterile 1.5 ml tubes.
Sequences for κ-opioid receptor protein were retrieved from the National Centre for Biotechnology Information (http://www.ncbi.nlm.nih.gov/) for sheep (accession AAY66995), zebrafish (accession NP_878306), rough skinned newt (accession AAU15126), mouse (accession NP_035141 and AAA56759) and guinea pig (accession AAA67171).
For example, in the current paper we have validated this assay on non-traditional amphibian (rough skinned newts, Taricha granulosa), reptilian (garter snakes, Thamnophis elegans), avian (house finches, Carpodacus mexicanus), and mammalian (coyotes, Canis latrans) species.
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