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According to the small molecular weight of firefly luciferase, 62 kDa [ 49] and rough estimates of time required for transcription and translation, images collected during the first 10-minutes after an overdose should adequately reflect luciferase made up to the time of imaging, and shorter exposures would provide even more conservative assays of expression.
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In applications in which it is necessary to distinguish one type from the other, one may examine the total section/test time of the students with lower RTE as a rough estimate of time pressure.
Self-reports are also only rough estimates of the time spent being physically active.
Janke et al. [ 48] used mitochondrial genomes of a total 35 species to obtain rough estimates of divergence times for Squamata and turtles, assuming a constant evolutionary rate between 2 reference points: the Synapsida/Diapsida split (310 Mya) and the Crocodylidae/Aves split (254 Mya).
We must emphasize that all of these rate estimates are approximate, based on rough estimates of minimum divergence times.
To investigate this further we estimated the mutation rate per year and per generation in mitochondrial DNA from the level of synonymous divergence at 4-fold degenerate sites, d4, for whole mitochondrial genome sequences from several pairs of animals for which we have well estimated divergence times, and a rough estimate of generation time.
We evaluated each tool based on a rough estimate of its time requirements to provide results for a "standard" restoration analyst (i.e., a Masters-level specialist trained in ecosystem service analysis, including GIS and/or economic valuation).
These studies highlight the importance of a stage in Beringia prior to the peopling of the Americas, and one of them even provided a rough estimate of the time the Native American founder population spent in Beringia using the number of diagnostic substitutions found in Native American mtDNA sub-haplogroups [13].
Seiler [ 17] also proposed a rough estimate of the time of divergence, based on the geographical distribution of the different reproductive types.
The τ value, which reflects the location of the mismatch distribution crest, provides a rough estimate of the time when rapid population expansion started.
We used these data to calibrate a relaxed molecular clock and to determine a rough estimate of the time of infection for each patient.
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CEO of Professional Science Editing for Scientists @ prosciediting.com