Our study showed that macrophage cell death induced by rough Brucella was dose-dependent since more cell death was observed with higher MOIs (Table 1, Figure 3).
In subsequent years, only rough estimations of population dose have been made based on the information on the number of CT studies and type of CT scans derived from yearly data collections of medical imaging practice in the Netherlands.
Water bottles were changed weekly and weighed to obtain rough estimates of the dose received by the animals.
A rough idea of the doses to be injected was obtained from the study of Sahu et al. (2011).
It was found that the level and speed of macrophage cell death were dependent on the infection doses of rough Brucella.
Treatment with B. adolescentis SPM0212 butanol extract caused RAW 264.7 cells to become larger and rougher in a dose-dependent manner, suggesting activation.
However, it is important to bear in mind that preclinical data can only give a rough estimate of the saturating dose of a mAb and that findings may not be directly translatable to the clinic.
Because all other factors were held constant, the postulated dose is a rough estimate.
Given many assumptions about uptake, metabolism, steady-state excretion, and other factors, biomonitoring measurements or concentrations can be used to provide a rough estimate of an internal dose, typically expressed as micrograms per kilogram per day.
Our study provides a rationale for a rough prediction of phenprocoumon maintenance doses.
