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In addition, as an excellent two-proton rotor probe, TM-V was able to report on viscosity in living cells and deeper tissues by ratiometric fluorescence imaging.
Sorption and drying processes were monitored in situ in polymer films by a fluorescence rotor probe, 4-tricyanovinyl-[N- 2-hydroxyethyl -N-ethyl]aniline (TC1), a solvatochromatic fluorescence probe, 4-tricyanovinyl-[N- 2-hydroxyethyl -N-ethyl]anilinepyrene.
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The Rotor-Gene SYBR Green PCR kit (Qiagen) was used for Real-Time PCR expression profiling of icaA[ 25] and gyrB (reference gene; [ 26]) using a Rotor Gene Probe PCR Kit (Qiagen).
The solid-state C and Si NMR spectra were obtained using a Varian VNMRS spectrometer operating at 100.56 mhz for C and 79.44 mhz for Si equipped with a 6 mm (outside diameter of the rotor) MAS probe.
For detection of viral RNA, we used 5 μL of RNA in a one-step real-time reverse transcription PCR upE assay (24 ) using the Rotor-GeneTM probe kit (QIAGEN) according to manufacturer's instructions.
In particular, it is shown that the shielding asymmetry factor, measured on samples with a regular shape and with a fixed orientation in the rotor, is a sensitive probe to ordered domains in the polymers.
5b, 7 The CLASSIC NMR strategy is applied here in C NMR studies of crystallization of m-ABA from dimethyl sulfoxide (DMSO), 8 using a Bruker AVANCE III spectrometer at the UK 850 MHz Solid-State NMR Facility (C, 213.82 MHz; 4 mm HXY probe; zirconia rotor suitable for spinning liquid samples; MAS frequency 12 kHz).
The resulting hydrophobic microdomains were characterized by both the hypsochromic shift of the wavelength emission band and the fluorescence quantum yield increase of a molecular rotor used as a fluorescence probe.
IL28B genotyping was performed using a specific customized TaqMan SNP-genotyping Assay (rs12979860; Applied Bosystem, Foster City, CA, USA) based on allele-specific labeled probes on a Rotor Gene 6000 (Corbett Research, Sidney, Australia).
The steady and unsteady flow fields at the outlet of the rotor are measured with a mini five-hole probe and a single-slanted hotwire probe.
Magic angle spinning (MAS) probe and 4 mm zirconia rotors spun by dry air were used.
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