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The results were counted as the total amount of net ipsilateral rotations per 120 min. One day after the drug-induced rotational test, rats were anesthetized with an overdose of pentobarbital (90 mg/kg, i.p., Mebunat, Orion Oyj, Espoo, Finland).
Rotational test to failure was performed with a material testing machine.
An apomorphine‐induced rotational test revealed no Parkinsonism‐like behavior in the NH treated groups.
The test rotor exceeded 350 Hz (21,000 rpm) in the rotational test, and whirl vibration was absent.
The rotational test with respect to the VSCC can be used as a tool for assessing vertical canal function.
Considering that the rotational test is only valid in animals with severe Parkinsonism, but time spent on the rotating rod correlates inversely with severity of Parkinsonism, our results indicate that a single exposure to low amounts of NH is effective in producing moderate Parkinsonism-like behavioral symptoms, possibly through a neurotoxic effect of this agent on the SN dopaminergic neurons.
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In the rotational tests, the rotor speed exceeded 380 Hz (22,800 rpm), and whirl vibration did not occur.
Although higher-end materials testing machines will enable torsional and/or rotational testing, simpler, less expensive systems rarely offer these capabilities.
For each focal length, several translational and rotational tests are performed, taking into account and analyzing different positions of the system inside the laboratory.
A biomechanical study by Zionts et al. [7] demonstrated that crossed pinning is more stable than lateral pinning in rotational testing as well as varus and valgus loading.
Dopaminergic functional activity in the striatum was assessed by [18F]FDOPA-PET, measurement of striatal dopamine (DA) and DA metabolite levels, tyrosine hydroxylase (TH) immunostaining, and methamphetamine-induced rotational testing.
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