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The optical system with two rotational stages and one linear motion stage is follow-up controlled to trace normal vectors, while the sample system with two rotational stages is fixed-command controlled.
This machine has two pairs of two-axis rotational stages with resolutions of 0.17 μrad and one linear motion stage with a resolution of 1 nm.
As described above, the neuroretinal flow described in our manuscript occurs in between the rotational stages.
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The sample was dipped into the medium-filled imaging chamber from the top and was translated along the z-axis with a linear stage M-404.1PD M-404.1PDInstrumente) and rotated with a rotational stage (M-660.55, Physik Instrumente).
The rotational stage is being used to rotate the sample with respect to the incident beam.
Instrumental controls drive a rotational stage with a fixed axis of rotation relative to a prism-based sample interface mounted on an inverted microscope stage.
In order to collect different views of a very complex object so to avoid shadows, the projection arm was attached to a rotational stage (Thorlabs Inc. Newton NJ) that was able to rotate around the sample in steps of 0.01 degrees, and a range of 0 to 200 degrees.
It is a conventional radiographic setup with a motorized rotational stage.
The scanning mirror system consists of reflective flat mirrors and a rotational stage.
The sample and the detector are placed on a rotational stage, which is driven by a stepper motor controller.
When nanometer scale X-ray microscopy is combined with a rotational stage, computerized tomography (CT) datasets can be collected.
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