Sentence examples for rotation overnight from inspiring English sources

Supernatant was incubated with 25 μl ANTI-FLAG M2 Affinity Gel (Sigma-Aldrich) with rotation overnight at 4 °C.

Following gentle centrifugation (600× g) for 5 minutes, beads were treated with protein-oligo mixture and incubated with gentle rotation overnight at 4 degree Celsius.

Cell lysate and antibody mixtures were incubated at 4°C with end-to-end rotation overnight, then with protein A or protein G agarose for two more hours.

DNA was extracted from bone in the same way, except for an initial decalcification of the bone shavings (∼10 50 mg) by incubation at room temperature, with rotation, overnight in ∼500 ul of 0.5 M EDTA pH 8.0.

Lysates (500 µg of proteins) were then incubated with rotation overnight at 4°C with 2 µg of rabbit polyclonal antibody anti-VDR, followed by an additional incubation for 2 h with protein G agarose beads.

Approximately 200 400 µg of protein cell lysates were incubated with 50 µL of Protein G/A beads (Miltenyi Biotec, Auburn, CA) and followed by sequential additions of 10 µL (2 µg) of cyclin D1 and Cdk 4 antibodies with end-to-end rotation overnight at 4°C.

Leave the vials containing the samples in the resin in the rolling rotation drum overnight.

For immunoprecipitations, primary antibodies were added to lysates and incubated with rotation for overnight at 4 °C.

After incubation at 4°C with rotation for overnight, the solution was added to 40 μl of protein A agarose and incubated for another 1 hours.

For HA affinity purification, detergent-treated microsomal membranes (1 mg total protein) was incubated with 20 μL of monoclonal anti-HA agarose slurry (containing 50% resin) equilibrated in PBS with rotation for overnight at 4°C.

The concentrated samples were resuspended with 400 μl coupling buffer and pH adjusted to 4.5 and then 500 μl of the 50%-slurry in coupling buffer was added to each sample and incubated at head-over rotation, RT overnight.