Your English writing platform
Discover LudwigSuggestions(2)
Exact(15)
The rotation assay of the F1-bead was conducted using the following procedures.
In ball rotation assay the lbRNAi flies were also significantly less efficient Fig. 4A and Video S10, S11.
Silverman et al. developed the rotation assay by tethering a flagellum on a coverslip and observing the rotation of the cell body [6].
A major difficulty in studying chemotactic behavior of V. alginolyticus is that the classical rotation assay [6], [7] that has been used successfully for E. coli cannot be reliably applied to this marine bacterium.
Similar to the rotation assay, our method is single-cell based and permits the study of behaviors of individual cells as well as the average behavior in a population.
The rotation assay allowed scientists to study a variety of problems ranging from a torque-speed relation [2], noises in flagellar motor [37], chemotactic responses [9], [12], and molecular interactions between the chemotactic regulatory protein and the motor complex [22], [38].
Similar(45)
Rotation assays were conducted in buffer C containing an ATP regenerating system consisting of 0.2 mg mL−1 creatine kinase (rabbit muscle, Roche Diagnostics) and 2.5 mM creatine phosphate (Roche Diagnostics).
The influence of Rac1 on cell-cell adhesion was determined by rotation aggregation assays.
To determine the cell-cell adhesion capacity, cell rotation aggregation assays were performed as described in [ 28].
Beausang et al. [84] indicated that the visibility of actin filament rotation in motility assays depends on the number of rigor bound heads providing a drag force to slow filament movement.
Protein G-Sepharose 4 Fast Flow (GE Healthcare, Piscataway, NJ, USA) was added to each sample followed by incubation for three hours at 4°C with rotation and then assayed for phosphatase activity.
Write better and faster with AI suggestions while staying true to your unique style.
Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com