During the catalytic process, a transesterification occurs, with tyrosine 723 of the active centre forming a covalent bond with a 3′ phosphate group, allowing the nicked DNA strand to rotate about the intact strand, and relieving thus by one turn the torsional constraint.
Starting with one β-strand aligned along the x-axis, a second β-strand, which is a copy of the first strand rotated by 180° along the z-axis, was placed next to the first strand along the y-axis separated by 10 Å.
By creating the single-stranded break, CtTopo I employ a mechanism called "controlled rotation" to relax supercoiled DNA, where one DNA strand rotates around the other [ 25].
On the C-C′ interface, each monomeric PKM2 adopts a rigid conformation and rotates alone with strand β20 (Fig. 2B D).
Since in the downstream DNA region the scissile DNA strand rotates around the intact one during the relaxation process, the high rigidity observed in the presence of the drug is in line with the blocking effect imposed by the drug itself.
The β-strands rotate to form a sheet tube that is a common character of these proteins.
DNA strands are then exchanged by a subunit rotation mechanism where one pair of synapsed subunits, together with their covalently-bound DNA strands, rotates 180° relative to the other pair (Stark et al., 1989; Dhar et al., 2004, 2009a, 2009b; Li et al., 2005; Bai et al., 2011).
Cut the steak in half, and note the grain: if the muscle is long strands, rotate the steak 90° and slice the rest across the grain in about 1/4-inch to 1/2-inch 5 to 10mm) slices.
He is also excellently served by Thea Sharrock's production which, with the aid of a set by Tom Scutt that resembles a gigantic, rotating cube, connects the play's multiple strands with exemplary clarity.
Continue curling 1 inch sections of hair by rotating the flat iron once and pulling along the strand.
