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This can typically be achieved by immersion techniques using a tub or other basin filled with ice cold water or via rotating cold ice towels over the body.
In this design, the rotating cold arm can have a narrower flexure compared to the flexure in the traditional single-hot arm device because it no longer needs to conduct an electrical current.
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Forty-eight hours after transfection, the cells were harvested and lysed by rotating in cold lysis buffer [10 mM Tris (pH 7.5), 100 mM NaCl, 1 mM EDTA, 1% Triton™ X-100, mammalian protease inhibitor cocktail form Sigma] for 1 h.
In melt spinning, a jet of molten metal is propelled against the moving surface of a cold, rotating copper cylinder.
Other variations of arcs mentioned in this study are pulsed, cold, rotating, buried, and rapid arcs.
The cold flow rotating mode is essentially hydrodynamic and corresponds to the well-known PVC (precessing vortex core) observed in many swirled confined flows.
Cell extracts were clarified by centrifugation for 15 min at 16 000 g at 4°C and processed for protein A/G precipitation on a rotating wheel in a cold room for 1 h.
Antibody at a concentration of 1 μl/10 μl beads was incubated at room temperature (RT) for 1 h in PBS containing 0.5% BSA, and unbound antibody was removed prior to IP. Beads were then incubated with chromatin in RIPA (1× PBS, NP-40 1%, Na-deoxycholate 0.5%, SDS 0.1%, sodium azide 0.004%) on a rotating platform in a cold room or RT as stated in the main text.
You can also tap the bearings off with a large cold chisel, rotating it and hitting it in different places to prevent it from binding as you go.
The rotary regenerator (heat wheel) is an important heat recovery equipment, which rotates between two cold and hot streams.
100 µl of the supernatant was incubated with 5 µl of normal rabbit serum and 20 µl of sepharose A and rotated in the cold room (4°C) for 2 hours.
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