Sentence examples for rot level from inspiring English sources

Importantly, in the clpX mutant, the Rot level was below this threshold level (the level was comparable to the Rot level in cells grown in the presence of 0.5 1% xylose).

Upon complementation of the clpX locus, Protein A and the Rot level were returned to wild-type levels (Fig. 4B).

This finding raised the question whether the relatively modest 2 3 fold reduction in the cellular Rot level is sufficient to abolish spa transcription.

Hence, we ruled out that the reduced Rot level in the clpX mutant is accomplished through an increase in RNAIII synthesis.

In post-exponential cells the Rot level was gradually reduced, reaching 75% and 50% of the exponential level at OD600 = 2.0 and 3.0, respectively.

After confirming that ClpX is required for full expression of Rot, we examined whether the 2 3 fold reduction of the Rot level can explain the dramatically reduced Protein A level of the clpX mutant [20].

The experiment clearly demonstrated that Protein A synthesis was dependent on the Rot levels (Fig. 3, central panel).

It is at the 80 μM and 100 μM ROT levels that the responses of the wild type and dwarf fibroblasts are the same.

To control the Rot-level, we employed a rot mutant that harbours a plasmid carrying the rot gene transcribed from a xylose inducible promoter [11].

As described above, the Rot protein level in the clpP mutant strain was comparable to the wild-type level, suggesting that the reduced rot transcription in clpX and clpP mutant strains is not reflected at the protein level.

The Northern blot revealed that both clpX and clpP mutant cells contained rot-transcript levels that were slightly reduced compared to wild-type levels.