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In general, Pearson correlation coefficients decreased as the root stage pairs became more distant to each other developmentally (Fig. 2a).
Therefore, it is reasonable to observe a synergistic effect formed in many bZIP proteins in specific stages, such as ZmbZIP50 and 68 in the V1 (vegetative 1, with first leaf fully extended) primary root stage and the VE (vegetative emergence) whole seedling stage.
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By contrast, tissue-specific differences identify functional specialisation of the different cluster root stages of P-starved plants.
Several genes of the auxin-mediated signalling cascade are differentially expressed in the analysed cluster root stages with higher transcript abundance in the root tip.
Cluster 1 is composed of genes with a low expression in the MCR and increasing expression in the two younger cluster root stages ICR and TCR, respectively.
We show marked differences in the transcriptional response across the various cluster root stages to adjust to phosphate limitation by increasing uptake capacity and adjusting metabolic pathways.
Several transcription factors (TFs) known to regulate root meristem function and lateral root initiation in Arabidopsis are also differentially regulated in the three cluster root stages of white lupin.
And the rooting stage is tricky, too.
A big, varied lineup is sensibly divided among a blues and roots stage (Buddy Guy, Isaac Hayes, Solomon Burke), a "jazz heritage" stage for the big names (Nancy Wilson and Ramsey Lewis, John Scofield, Brad Mehldau, Blind Boys of Alabama), and a locals stage.
They modified gravireaction several times during the course of their development: a first horizontal-growth stage when they grow in the cortex of primary roots (stage I), a sloping-down growth stage from their emergence to a length of about 1 mm (stage II), a second horizontal-growth stage from a length of about 1 mm to that of over 4 mm (stage III) and a curving-down stage thereafter (stage IV).
But it has also returned to its civic roots: staging patriotic events, campaigning against corruption, participating in urban renewal, delivering humanitarian aid – much to the adulation of the local population.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com