Serial sections of the aortic root were mounted on masked slides.
Later on, roots were mounted in 0.05% trypan blue in lactophenol for microscopic examination (Michal Johnson et al. 2011).
The roots were mounted on a microscopic slide in 50% glycerol in 1X PBS; 0.3-μm thick longitudinal optical sections were acquired on a Zeiss LSM-510 Meta confocal microscope using 63X oil immersion (NA 1.4) and were further projected to obtain the image of 2 to 3 μm total thickness.
The rejuvenated institution, now called The Contemporary, will return to its nomadic roots by mounting exhibitions at several as-yet-undetermined locations throughout Baltimore, instead of staging them in just one building that viewers have to deliberately decide to visit.
For confocal microscopy, roots were mounted in propidium iodide (PI; 20 µg/mL in distilled water).
The roots were mounted in water and then observed by fluorescence and differential interference contrast (DIC) microscopy.
The roots were mounted vertically into Eppendorf tubes partially filled with acrylic resin.
The L4 and L5 roots were mounted on hook electrodes in continuity for recording of the afferent volley.
The roots were mounted in a solution of chloral hydrate:glycerol:H2O 8:3:1 and left overnight at 20°C to clear.
For live cell imaging, wherever possible, roots were mounted in a propidium iodide (PI) solution (0.02 mg/ml) for counterstaining the cell walls.
For observation and documentation, GUS-stained roots were mounted on microscope slides or in small wells, and photographed with either a Leica DMLB light microscope and a Nikon Eclipse 90i (Leica Microsystems GmbH, Wetzlar, Germany; Nikon Corporation, Tokyo, Japan), or a stereomicroscope (Leica MZFLIII, Leica Microsystems GmbH) equipped with a Nikon DS-Fi1 camera.
