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In addition, upon a 3 day induction during the exponential phase, a trp5 ASB1 hairy root line produced 1.8 times more tryptophan (specific yield ca. 3.0 mg g−1 dry weight) than the trp5 hairy root line.
These transgenic hairy root lines produced higher quantities flavones (i.e., baicalin, baicalein, and wogonin) than the control hairy root line.
For out-of-plane gusset joints, the effective notch stresses along the weld root line depend significantly on weld size and weld penetration size.
The time course for changes in dry weight of fastest growing hairy root line 5 (H5) during the 8-weeks culture period is shown in Figure 4.
When TDC is ectopically expressed together with trp5 and ASB1, the induced trp5 ASB1 TDC hairy root line accumulates tryptamine up to 14-fold higher than the uninduced line.
The time course for changes in the fastest growing hairy root line 5 (H5) during the 8-weeks culture period showed the product stabilizes at the 5-weeks period.
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All the tested transgenic hairy root lines showed higher antioxidant activity than the control.
Recombinant hairy root lines overexpressing VoFPS or VoVDS were generated and compared to control cultures.
The transformed hairy root lines were confirmed by PCR using rolB and rolC gene-specific primers.
Metabolic analysis of RNAi-AH1 hairy root lines showed a significantly increased accumulation of miltiradiene compared to the control lines.
Lane M Molecularr weight marker; Lane Nt: non-transgenic line; Lane No. 1 5: transformed hairy root lines; P: positive control.
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