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J2 effector proteins are injected into the root, dissolving plant cell walls and driving formation of a metabolically active, multinucleated feeding site known as a syncytium [ 3].
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For the aqueous extraction, eight hundred grams (800 g) of the powdered roots were dissolved in 5 l of distilled water and kept for 72 h at 25°C, and occasionally stirred.
Check that the roots are dissolved and it's getting loose to give space to a permanent tooth.
As the rooting zone becomes devoid of dissolved phosphate, root proliferation or enhanced reliance on mycorrhizal associations and root exudates become necessary to maintain P supply [43].
The methanolic root extract was dissolved in DMSO at 50 mg/mL as a stock solution and diluted to the required concentrations with fresh medium immediately before use.
Heating to 75 °C until kudzu root starch was dissolved completely, then α-amylase was added for liquefaction.
The root extracts were dissolved in methanol/water (95 : 5, v/v) (2.5 mg DW mL−1).
The root extracts were dissolved in methanol (1.0 μg DW mL−1) and filtered through a 0.22 μm membrane of a nylon filter.
All other chemicals and reagents were obtained from Sigma-Aldrich Chemical Company, St . Louis MO, U.S.A. Different concentrations of C. sieberiana root bark extract (dissolved in water as vehicle) were administered by oral gavage to the different animal groups (six animals per group) at 12 h intervals in a total volume of 2 mL for 7 days.
The ginger root will not dissolve, of course.
Gardea-Torresdey et al. [36] proposed that the roots absorb the dissolved minerals by capillary action and subsequently reduced them to metal nanoparticles.
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