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This mechanism has been studied extensively both in vitro and in vivo with different cell types, such as leukocytes or hematopoietic stem cells and is believed to rely on adhesion molecules and cytokine receptors, consisting of a rolling interaction with the endothelium, followed by firm adhesion and transmigration into the surrounding tissue.
Only a few bonds need to be present at any time in order to maintain a rolling interaction.
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The binding through L-selectin is dependent on sufficient shear stress above a critical threshold, to promote and maintain rolling interactions [28].
MSCs modified with SLeX exhibit significantly improved rolling interactions with a velocity of 8 μm/s as compared to 61 μm/s for unmodified MSCs at a shear stress of 0.5 dyn/cm2.
However, under flow conditions, rolling interactions via P-selectin remain unaltered.
However, they exist natively in a low affinity state and can only aid in supporting rolling interactions.
The main physiologic function of selectins is to allow high-affinity rolling interactions between leukocytes and the endothelium under flow, mediating signal transduction and firm adhesion.
Using the parallel plate flow chamber, we examined rolling interactions of undifferentiated HL-60 cells for two different incubation concentrations of P-selectin: 0.5 and 1.5 μg/ml.
We also examined the effect of DMSO (e.g. 48, 72 and 96 h exposure) in order to ascertain its effect, if any, on HL-60 rolling interactions.
The changes in adhesion molecule expression were accompanied by a significant reduction of firm adhesion to 54%, whereas rolling interactions remained unchanged.
Using the parallel plate flow chamber, we examined the rolling interactions of HL-60 cells for two different incubation concentrations of P-selectin: 0.5 and 1.5 μg/ml.
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