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Amplified DNA was not recovered from the rodent sample CE155, which had the lower antibody titer (Table 3).
Despite performing several PCRs, we could not amplify the Ypc locus from R. rattus rodent sample R05 from Antanabe (Table 4).
However, within this locus, a new, and to our knowledge, Madagascar-specific spacer c12 was detected in R. rattus rodent sample R16 from Ambarakaraka and in a Bealanana-013 sample from 2010 (Table 4).
We found a rodent sample (F0910) with canSNP and MLVA genotypes identical to an oropharyngeal clinical sample (F0898) (Technical Appendix Table 1), a finding consistent with water contamination that originates from animal sources.
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None of the 1170 cattle samples or 166 rodent samples analyzed was positive.
Significant differences in the relative prevalence of anti-ANDV antibodies in rodent samples also were found across the three ecoregions.
INTS7 was constantly detected in all the rodent samples examined, as was the case for G3PDH (Fig. 6B and C).
Rodent sampling occurred in "Spring" (May and June) and "Fall" (late August and September, October) of 2003, 2004 and 2005, during 15 consecutive day periods that coincided with the new moon.
Our measurements of ACC1 and ACC2 transcript levels in rodent samples agree with published data [14], [23] showing high levels of ACC1 in adipose, high levels of ACC2 in heart and skeletal muscle tissues, and higher levels of ACC1 than ACC2 in liver.
No variants could be assembled in the rodent samples.
Preliminary and limited rodent sampling has not produced any hantavirus antibody positive samples.
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