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This procedure allowed us to mildly regenerate the chip surface by removing the biotinylated SNAP tag fusion protein and thus enabled us to use one and the same chip for different SNAP tagged septin rod preparations.
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The N-terminally fused S tag to the Cdc11 subunit of the rod allowed us to control the integrity of the captured rod preparation by injecting the antibody against the S tag in saturating concentrations.
I nod in agreement and start to lift my rod in preparation for another cast when a salmon crashes the surface, grabs my fly, then goes berserk, unnervingly splashing and tail-slapping all over the once serene pool.
Standard instrumentation was used to align and position the guide rod for the preparation of the femoral head using the lateral cortical pin and out-rigger.
We investigated the functional relationships between chromatin compaction, distribution of histone modifications and location of RNA polymerase II in intact murine rod photoreceptors using cryo-preparation methods, electron tomography and immunogold labeling.
Figure 2 Preparation of the Au rod @pNIPAAm-PEGMA nanogel.
The whole preparation was mixed with a rod and rotated at 120 rpm.
Only when the initial yield of rod-shaped cardiac myocytes was >50%, preparations were considered suitable for experiments.
Similarly, by preparation of rod-shaped specimens the same group has provided 3D structural correlations and quantitative compositional mapping at a resolution of approximately 7 nm using tandem cells [71].
Via aggregation, preparation of rods or core/shell geometry they can be made to strongly absorb near IR-light [ 24, 25 ].
Neither can it be a component of the kinetoplastid-specific paraflagellar rod, which was present in the trypanosome flagellar preparation.
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