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We validate its utility using RNA interference knockdowns of the paraflagellar rod gene PFR2 of L. (Viannia) braziliensis.
In general, however, the effects of Nr2e3 and Neurod1 mutations on rod gene expression are modest [18]-[20] [18]-[20] not shown).
Without exogenous T3 added to the system, a striking ventralized pattern emerged that resembled both early Dio2 RNA expression, as well as the later expression of the rod gene, rhodopsin [71] (Fig. 6A-A').
Lightning rod: gene loss.
One additional gene, p27Kip, was added to the hypothesized rod gene network based on its interaction with two candidate genes.
These new links in the resulting rod gene network offer a rich source of hypotheses that can help focus the experiments at the bench.
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Hot Rod genes allow translating ribosomes and E. coli RNA polymerases to maintain coupled translation and transcription at maximal rates.
Nrl and Nr2e3, in contrast, are rod-specific and are required for activation of rod genes and repression of cone genes [13], [15], [17] [20].
NR2E3 has been shown to regulate gene networks involved in photoreceptor development and function, and loss of Nr2e3 causes mis-regulation of cone and rod genes [25] [29].
Following immunohistological and real-time PCR analyses, we further observed that decreased expression of rod genes in Rpe65-deficient mice was rescued in Rpe65−/−/Bax−/− mice.
This latter gene is interesting in light of the critical role that retinoic acid signaling plays in dorsoventral patterning of the chick and zebrafish retinas [99], [100], as well as the expression of rod genes, including rhodopsin [101], [102], [103].
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