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Further studies with a focus on nucleation kinetics are now needed to determine how this process can be improved (yield, crystal size distribution, robustness) scaled-up and accelerated: for instance, by seeding the solution with pure urate oxidase crystal fragments or by concentrating the solution to increase the nucleation rate.
The calculated assay Z′ factor of 0.71 ± 0.03 indicated an excellent robustness for scaling up to HTS formats.
If evidence is gathered from several areas and sources, the greater robustness of information helps provide confidence that a given initiative can be scaled up further or adopted elsewhere.
Could it be scaled up?
The Obamas scaled up.
Can learning be scaled up this much?
And how can they be scaled up?
And the method scaled up nicely.
How will the project be scaled up?
"They're interested in scaling up".
But scaling up will be challenging.
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