Exact(4)
As shown in Fig. 4A, KaiCTST, KaiCaST, and KaiCnST robustly label with 32P at 30°C for 24 h presumably because S431 and T432 are available for phosphorylation in these proteins.
Currently, no antibodies are available that robustly label the presynaptic calcium channel in vivo.
Oligopaints robustly label chromosomes both in tissue culture cells and whole-mount tissue preparations and can be generated using standard molecular biology techniques and equipment at a price well below the cost of commercial FISH probes.
Metabolic oligosaccharide engineering-based incorporation of 1,3,4- O-Bu3ManNAz into the glycoconjugates of various cell lines including SW1990 was recently investigated [ 34], suggesting that 50 μM of analog was an adequate concentration to robustly label sialoglycans without inducing any noticeable cytotoxicity, growth inhibition, or other "off-target" effects.
Similar(56)
Lifeact-mEGFP robustly labels actin microfilaments, particularly in the apex, in both moss protonemata and pollen tubes.
Nevertheless, the results confirmed F109azF-CCR5 as the most robustly labeled mutant in the screen.
(I – N ) Early induction robustly labeled cells in the left ventricle and atria of double transgenic embryos.
Note the hollow tip of the comet, which is not labeled by GFP-PHPLCδ, even though this probe robustly labels the plasma membrane encapsulating the comet.
(C – H ) Early induction labeled few reporter cells in the retina of double transgenic embryos, however later induction robustly labeled cells within the retina.
In the neocortex, entorhinal cortex (ERC) and hippocampus, 6-CN-PiB robustly labelled compact/cored plaques, while diffuse/amorphous plaques were less prominently labelled (Fig. 1).
In the hippocampus, the dentate molecular layer, hilus, mossy fibers, CA1 neuronal cell bodies and apical dendrites were all robustly labeled while the CA3 was moderately so.
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