The GC/Quadrupole-Orbitrap robustly generates, transmits, manipulates, and detects EI/CI-generated ions supplied by a GC in four modes: full scan, SIM with quadrupole isolation, "all ion fragmentation -MS/MS with beam-type CAD in the HCD collision cell, and MS/MS with both quadrupole isolation and beam-type CAD.
The projection image generation algorithm [11] was proposed to design and fabricate a complex 3D scaffold, which automatically and robustly generates 2D projection image data.
In summary, OSCAR methodology combines PCR and Gateway® technology to rapidly and robustly generate precise deletion constructs for fungal ATMT and homologous gene replacement.
Only primary DG cells robustly generated DG neurons (80% NeuN and Prox1-positive cells at 6 weeks), substantially repaired the damaged DG, and formed glutamatergic projections to the target CA3 region.
As previously reported SFEB robustly generated heterogeneous cultures containing neural ectoderm in the form of neural rosettes after plating EBs in adherent plates, referred to here as EB-rosette [31], [32].
By developing the assay in a clonal cell line in which stable lines were first generated from the receptor subtype followed by plasmid transfection, or transduction with a viral vector containing the recombinant DNA encoded sensor, one can robustly generate previously established receptor subtype clones containing the FRET based sensor.
In contrast to glutamate disruption from any source, both motivation and hedonic impact are robustly generated together by GABAergic inhibition of neurons in medial shell, produced by inputs from neighboring medial spiny neurons and other intrinsic NAc neurons, or from GABAergic inputs from other subcortical structures [1], [56].
While single sorted stem cells grow inefficiently in culture, stem cell/Paneth cell doublets robustly generate mini-guts.
These cells very quickly adopt an oligodendrocytic fate and robustly generate myelinating cells as efficiently as pOPC do.
The authors demonstrated that this fragment is robustly generated when MMP-2 is not complexed with its endogenous inhibitor TIMP-2.
