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Here, we will highlight three ambitious novel therapeutic approaches for this condition, each robustly driven by primary neuroscience.
Reprogramming of somatic cells to naive pluripotency can be robustly driven by the combined action of transcription factors and culture cues.
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We also employed the model organism Drosophila melanogaster as a readout for activation of ALK by FAM150A and FAM150B, showing that FAM150 proteins are able to robustly drive human ALK activation when ectopically coexpressed in the fly.
These stimuli robustly drive pitch-responsive brain regions while producing minimal cochlear and earphone distortion, and will hopefully aid fMRI researchers in avoiding distortion confounds.
However, we found that several Otx2 DHSs robustly drive expression of the GFP reporter in the retina.
In contrast, when we generated otd −/− neuroblast clones in the LALv1 lineage (identifiable by the loss of Otd staining ventral to the antennal lobe; magenta dotted lines in Figure 9N) the Cha7.4-Gal4 drobustlybustly drove reporter expression in the transformed otd −/− LALv1 lineage (magenta dotted lines and insets in Figure 9M P).
Three Δ3 4-hiPS cell lines propagated robustly under puromycin selection driven by the EOS-pluripotent reporter and were subjected to further characterization.
Similarly, using a retroviral system that allowed expression of SYT-SSX2 in U2OS cells (human osteosarcoma) at levels similar to those detected in primary synovial sarcoma cells (Syn1 cells- Figure 1A; in synovial sarcoma, SYT-SSX2 expression is driven by the robustly active promoter of SYT), we were able to detect substantial colocalization of SYT-SSX2 with the polycomb protein Bmi1 (Figure 1B).
A single-cell model with a Hill coefficient of repression as low as 0.7 was still able to function as the building block of a robustly oscillating cellular network when driven by the neuropeptide mean-field.
Previous studies in neuronal cell cultures showed that intracellular transport of short MTs occurs robustly in neurites and can be driven by mitotic kinesins (Baas et al., 2006).
We have previously shown that luciferase expression driven by this construct is robustly upregulated in response to hypoxia in a range of cell lines (Cowen et al. 2004, Chadderton et al. 2005).
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com