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As expected, FGF-iPSCs robustly display X-inactivation upon differentiation, demonstrating that the cells are capable of X-inactivation (Figure 4C).
Furthermore, regional differences in NSC behavior have been described (Seaberg et al., 2005; Seaberg and van der Kooy, 2002): From embryonic development to adulthood, the anterior lateral ventricle contains NSCs that robustly display the stem cell characteristics of self-renewal and multipotentiality.
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A second decision-making style more robustly displayed was the C.E.O.
In particular, we show that a network combining an activation threshold and strong negative feedback also robustly displays such bimodal population behavior due to cell-to-cell variability in protein expression levels.
We demonstrate here that engineered protein motifs can be robustly displayed to mammalian cells in vitro in a highly controlled manner using a soluble protein scaffold designed to self assemble on a gold surface.
We believe that our data robustly show that CLCA1-mediated currents display essentially the same Ca2+-, voltage- and Cl– dependence as those described in the literature for Ca2+-activated Cl– channels, and in particular TMEM16A.
Splice variants possessing the exon-5-encoded motif at the amino-terminal domain (ATD) of the GluN1 subunit are known to display robustly altered deactivation rates and pH sensitivity, but the underlying mechanism for this functional modification is largely unknown.
The peripheral MP neuromodulin displays robustly with packaging by conventional M13-KO7 helphagehage.
They reported that there was a thin line between level of expression and tamoxifen control as robustly expressing lines displayed rapid and complete recombination, but might have leakiness, whereas those that had tight regulation showed low expression.
The upper four plots in each column display the robustly estimated remote reference TFs for the indicated site.
Only a sub-fraction of robustly VASA-positive cells displayed clear PLZF expression (Fig. 7A, white arrows).
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