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However, in the current study, we used a larger number and variety of non-metastatic tissues to illustrate the fact that the metastatic signature is robustly different from all the non-metastatic conditions that one might use for comparison.
To identify strains that are robustly different from N2, we bootstrapped the phenotype barcode data and identified strains different from N2 as ones that remained outside the N2 cluster in at least 90% of the bootstrap clusters.
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A robustly recovered matrix was in four cases significantly different from the actual covariance matrix and overestimated variances by 72 and 24%%.
A robustly recovered matrix was in four cases significantly different from the actual covariance matrix and overestimated variances by 72 and 24%%.> -wrap-foot> Acovarianceriance matrices are based on a sample size of 50.
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It is possible that the cell state of the cultured MG/MΦs in our system may be different from in vivo MG/MΦs after SCI and that other micro-environmental factors, such as astrocyte activity, may be required to robustly elevate IL-10 secretion from MG/MΦs.
Real-time PCR using brain hippocampal glio-neuronal cultures of WT, Nrf2-KO, and Keap1-KD mice showed that NOX2 mRNA levels are robustly elevated in Nrf2-KO (~7-fold increase compared to WT; p < 0.01; Fig. 5A), whereas NOX2 mRNA expression in Keap1-KD was not different from WT cells.
The ability to robustly compare different clinical proteomics datasets would facilitate the integration of datasets from proteomics and genomics and transform the field of clinical proteomics.
This result holds robustly for different biological and synthetic networks.
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