After seven days of growth, robust seedlings were potted in soil and grown in a growth chamber at 22°C and 60% relative humidity under a 9-h photoperiod.
Although growth at 15 °C did not appear to enhance the sucrose dependence of pex4-1 (Fig. 4a), we found that pex4-1 seedlingrownown at 15 °C were more IBA resistant than seedlings grown at 22 °C (Fig. 4b, Additional file 1E) whereas wild-type seedlings displayed robust IBA responsiveness when grown at 15 °C.
Seedlings that germinated exhibited robust phototropic curvature.
In an attempt to identify robust QTLs for salinity tolerance at the seedling stage using a novel donor, the following activities were carried out.
Subsequently, we used the simple, robust ABA-inhibition of seed germination and early seedling development assays to examine the ABA sensitivity of these mutants.
By using the accepted and robust ABA-mediated inhibition of seed germination and early seedling development assays, we demonstrated that none of the gcr2, gcl1 and gcl2 single, double and triple mutants displayed ABA insensitivity (Figure 4).
These are most conspicuous in early seedling development, as initial responses to light are rapid, robust, and have been well characterized [ 5, 24- 26].
Seedlings, juvenile and adult phases of O. flocktoniae were the most robust stages in the life cycle of this species.
Our previous microarray study on smoke-exposed maize seedlings showed that smoke treatment results in a distinct, although not robust, change in the gene expression pattern.
Information about seedling morphology provides a new data set that will help contribute to a more robust discussion of not only seed plant phylogeny, but also to our understanding about developmental processes that occurred in fossil plants after embryo formation and during seedling development.
