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In order to mimic the known bone-anabolic pharmacokinetic (PK) profile of s.c. administered PTH, such molecules must trigger sharp, transient and robust release of PTH.
Longer processing time and/or faster mixer speed was needed to achieve robust release with compositions containing DC2 compared with those containing CR.
However, robust release was also achieved when using HPMC DC2 at high ibuprofen content, even though it took slightly longer time to reach the steady state of the process.
It is of note that intra-mPOA administration of kisspeptin-10 evoked a robust release of LH, thus it can be presumed that the antagonist delivered into this region also has access to the kisspeptin receptor.
Interestingly, NK cells treated with BAT3-depleted exosomes failed to produce TNF-α and IFN-γ, whereas a robust release was observed with control exosomes (si-c) derived from control si-RNA transfected cells.
In this context, the low level activity observed during the second 24 hr period a.p. in non-diapausing animals may represent a leakage activity that precedes the full blown proliferative activity that initiates around 48 hrs a.p. in response to a more robust release of hormone.
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Additionally, the model was robust with release kinetics being insensitive to changes in pH, ionic strength, viscosity of the release medium, and revolution speed of the donor cell.
Robust ibuprofen release was obtained faster when HPMC CR was used.
Unfortunately, due to its small size, its development as a therapeutic requires a robust controlled release system.
We have established a preparation in which we assessed stimulation-induced, reversible and robust CGRP release from single mouse medullary brainstem slices.
Developing robust, predictive release models requires systematic, quantitative characterization of these complex drug delivery systems with respect to the physicochemical properties governing the driving force for release.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com