Sentence examples for robust polymerase from inspiring English sources

Besides, viruses with robust polymerase complexes might be more capable of creating adaptive mutations under a selection pressure.

Unlike any other cloning method, CPEC relies solely on the simple and robust polymerase extension mechanism to clone individual genes, libraries, or multiple fragments.

Importantly, this adjustment of the assay conditions not only revealed the robust polymerase activity of IAV Pol complex, but also enabled us to investigate the enzyme fidelity of IAV Pol.

The reactions also contained 1X Buffer, 2.25 mM Mg, 0.2 mM of each primer, 0.2 mM dNTPs, 0.5 U KAPA2G Robust polymerase (Kapa Biosystems Inc ,Boston, Massachusetts).

The robust polymerase chain reaction (PCR) amplification efficiency and high polymorphic potential of GNMS markers over genic coding and random genomic microsatellite markers suggest their immediate use in efficient genotyping applications in rice.

In addition to being a robust polymerase for the amplification of large genomic regions, this polymerase also has an error rate of approximately 8.7 x 10−6 per base incorporated, and therefore introduces far fewer errors than PacBio chemistry does.

Within this scope, as fast and robust methods, polymerase chain reaction (PCR -based techniques are PCR -basedin mostechniquesries due to their efficiency are spreferredy.

Based on this technique, a simple and robust multiplex polymerase chain reaction/restriction fragment length polymorphism (multiplex PCR/RFLP) assay was developed to determine simultaneously three to four informative SNPs (IL-1β/+3954, IL-1β/−511 and IL-1Ra/9261 or IL-1α/−889 IL-1α/−889, IL-1β/−310 and IL-1β/581000 SNPs) in the IL-1 gene complex.

Here, we demonstrated that a H1N1 with a robust vRNP polymerase could induce hypercytokinemia in primary human cells.

The introduction of the low-temperature PCR system with a robust DNA polymerase, which does not require sample purification or DNA extraction, has simplified the process to direct DNA sequencing procedure, making rigorous validation of HPV genotyping in a clinical laboratory economically sustainable [ 26- 28, 30].

We recently reported that during HSV-1 infection when viral transcription is robust, cellular RNA polymerase II (RNAP II), and specifically the serine-2 phosphorylated form of the C-terminal domain (CTD) found in elongating transcription complexes [8] [10], undergoes ubiquitination and proteasomal degradation [11].