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Interpretation of how different call designs have evolved has been hampered by the lack of a robust phylogenetic framework.
Many key aspects of animal evolution can be reconstructed by comparing living species within a robust phylogenetic framework.
In plants, the Oryza genus provides such a unique opportunity to investigate various aspects of gene and genome evolution with the availability of a robust phylogenetic framework [31, 97], rich genomic resources [5, 49], and a near-perfect reference genome [41].
However, a robust phylogenetic framework for the family is currently lacking.
This primarily requires a robust phylogenetic framework for the entire subtribe, which is currently lacking.
In the present study we attempt to establish a robust phylogenetic framework for the relationships of the Lasius subgenera.
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Comparison of these data for the island endemics versus their congeners on the continent is beyond the scope of this paper due to a lack of available robust phylogenetic frameworks (with exceptions in Sonchus and Crambe) and a reliable data for clock calibration.
Further investigation of reproductive systems of Psychotria on oceanic islands using robust phylogenetic frameworks would provide new insights into plant reproduction on oceanic islands.
However, inferences about morphological character evolution in dinoflagellates depend on a robust molecular phylogenetic framework, especially at the deepest levels that relate the major subgroups (i.e., "orders").
This study provides a robust Ursidae phylogenetic framework for future validation by additional independent evidence, and also has significant implications for assisting in the resolution of other similarly difficult phylogenetic investigations.
In order to calculate speciation rates and assess speciation modes, a robust and detailed phylogenetic framework is required in order to determine evolutionary relationships and timing of cladogenetic events [41] [42].
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