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After about six weeks, we selected from each donor a well-proliferating culture derived from the lowest initial PBMC number that had led to robust outgrowth.
The basal layer consists of myoepithelial cells (MYOs) and mammary stem cells (MaSCs), the latter characterised by their robust outgrowth activity in the cleared fat pad transplant assay.
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Using limiting cell dilutions (10k C57Bl6 MECs) and scoring both the robust outgrowths and partial outgrowths, we confirmed that Lrp5-/cells have less stem cell activity.
This allows for more robust neurite outgrowth on the nanotopographical versus the 2D ECM.
Several related human IgMs that bind to the surface of neurons support robust neurite outgrowth (Warrington et al., 2004; Xu et al., 2011b).
Robust axon outgrowth is elicited by the prosp-NH, with significantly more axons projecting from the proximal than the distal face of [L] explants (Fig. 2E,F; supplementary material Table S1).
Additionally, the impaired outgrowth and proliferation of 3AKO LCLs complicates the analysis [10], [11], in contrast to the robust growth of 3BKO and wtBAC LCLs.
Robust growth.
Control organotypic slice cultures grown on collagen coated membranes exhibited robust ventral neurite outgrowth after 2.5 weeks in culture.
Our results indicate that axon extension in the pharynx appears to be robust and its proximal outgrowth and distal outgrowth are mediated by two different mechanisms.
Robust, rowdy growth.
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