Mutant osmr3, the most robust mutant, was converted in its markerless derivative (osmr3f).
As a consequence of its improved stability, only the robust mutant scFv genetically fused to the ribonuclease rapLRI mediated cytotoxicity towards tumour cells and recognised its native target antigen when displayed on phage.
Columns from third to fifth represent the fraction of timing-robust mutants in each model.
In the phase transition from the G2 phase to the M phase, the Mangla, et al.'s model was found to be vulnerable to mutations (Table 6), and the fraction of timing-robust mutants holding the cell cycle property was much smaller than in the proposed model.
On account of its robust stability, mutant DS255 would be a competitive candidate in practical applications of chiral chemicals synthesis, biofuel cells and glucose biosensors.
The transheterozygous genetic interaction we observe between capulet and khc as well as the robust double mutant phenotype that produces actin rods in dendrites suggests a strong cellular interdependence between these two molecules (see model Figure 7).
I-CXCL11 binding was robust among all mutants, except for the D112N mutant, which bound I-CXCL11 at detectable but significantly reduced levels compared with WT CXCR3.
This study also found that the E897K mutation at the AF-2 domain led to a more robust rescue of mutant AR phenotypes than K720A, again demonstrating that the ability of the AR AF-2 domain to bind FxxLF-containing cofactors may be important for SBMA pathogenesis (Nedelsky et al., 2010).
To obtain a robust comparison between mutant proteins, the number of parameters was kept at a minimum.
