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We empirically used 0.6 as the cutoff value of membership robustness and took any cluster with at least five robust members to define the gene signature.
The clustering results were further merged to identify the consensus clusters and their robust members.
A consensus clustering approach was employed to determine the proper cluster numbers and the robust members within each cluster by using R clusterCons package [ 40].
For each cluster, a gene signature was defined as the median of the fold changes of the robust members relative to the normal controls.
The feedback loop that the system necessarily possesses in the homeostatic state, ensures that the robust members of the loop are themselves regulated and kept close to their threshold values.
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It was the largest and most robust member of its genus, and the hackles were longer and covered a larger area than in other blue pigeons.
Thus, a robust member, by definition, should have a high score (i.e., close to 1).
In order to ensure robust analysis, members of the research team reviewed emerging themes throughout the analysis phase.
Therefore, we focused on the remaining 197 up-regulated genes and clustering produced five clusters with robust gene members (i.e., cluster A-E; Additional file 2: Figure S2C and S3; also see Methods).
This study offers considerable prospects of expanding the structural repertoire of β/α Ant-Pro motif, which has been described earlier to assume right-handed helical architecture displaying robust nine-membered-ring closed network of hydrogen-bonding interactions, into γ/α 2-Amb-Pro motif.
The line was met with prolonged and robust applause from members of the audience on both sides of the Democratic-Republican divide, although at least one member of the US Senate remained unimpressed: Earlier in his address, Obama took a slightly more veiled swipe at Trump.
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