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Moreover, Q-PCR expression levels for the low-expressing orthologue BraXTH24 and three high-expressing orthologues for COPPER CHAPERONE for SOD1 (CuCHAP, Bra036642), PECTIN METHYLESTERASE (PME61, Bo4g108130), and CCR2 (Bra035150) were consistently higher in the two less robust lines compared with robust lines (Fig. 8).
Two redox genes showed highest overall expression in K-5-8 leands and lowest in O-3-7-5 leaves, while one redox gene and three cell wall genes were consistently higher in the two less robust lines compared with the two robust lines.
From these four robust lines that most closely mimicked wild type growth, a single line that was homozygous for the transgene was selected as a representative line for further study (Fig. 2F; line 1039).
To be consistent in selecting representative lines, a single line homozygous for the CoYMVp::cSUC2 transgene was selected for further study from these four most robust lines (Fig. 2G; line 1070).
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CEO of Professional Science Editing for Scientists @ prosciediting.com