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The next phase in the integration of design into healthcare is a move from individual successes like the Kit Yamoyo and the insulin pen to a more formal, robust incorporation of design as a fundamental component of medicine.
All known in vivo substrates of Ypk1 (Fpk2, Fpk2, Gpd1, Orm1 and Orm2) display robust incorporation (as judged by autoradiography) in this in vitro assay (Roelants et al., 2010, 2011; Lee et al., 2012b).
During the recovery from FCCP, brisk mitochondrial biogenesis occurred as indicated by the robust incorporation of newly synthesized (green) MitoTimer protein (Fig. 6A); by comparison, there was much less MitoTimer protein import in the vehicle control cells.
Previous studies showed that when sialic acid or its analogues were used directly as metabolic precursor, millimolar concentrations were required to achieve robust incorporation and metabolic remodeling of cell-surface sialylated glycoconjugates.
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We observed robust incorporation of GalNAz into glycoproteins, indicating that C. elegans' metabolic enzymes are tolerant of this azidosugar.
In contrast, pRb-deficient MC3T3 cells showed robust BrdU incorporation in both the nodules and their periphery (Fig. 1F, top right, arrow indicates bone forming nodule).
After peaking at 7 dbc, pRb-expressing osteoblasts showed a significant reduction at 14 dbc, a time point at which pRb-deficient MC3T3 osteoblasts still showed robust BrdU incorporation.
Embryos incubated with BrdU from 1 to 10 som showed robust BrdU incorporation at the midline (Fig. 2E), and a weaker signal overlying the presumptive LPM (red arrows, Figs. 2F and 2G).
Affinities and maximal velocities differ quantitatively, but the presence/absence of each property is robust to incorporation of uncertainty about the ancestral sequence (see Figure 1F ).
With an evolving field of possible targets and ligands, the challenge for the engineers then is to create a robust mechanism for incorporation of these new ligands to a polymeric delivery vehicle.
Extending our analysis to the dynamic model of Jofre-Bonet and Pesendorfer (2003) shows that the main crisis effect is robust to the incorporation of the option value effect of winning.
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