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The cultivation efforts within the National Alliance for Advanced Biofuels and Bioproducts (NAABB) were developed to provide four major goals for the consortium, which included biomass production for downstream experimentation, development of new assessment tools for cultivation, development of new cultivation reactor technologies, and development of methods for robust cultivation.
Genetic determinants for virulence of Cryptosporidium spp. and reasons for emergence of virulent subtypes are poorly understood because of availability of only limited genomic sequence data and lack of robust cultivation systems and genetic manipulation tools (13 ).
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To facilitate scaled photosynthetic biomanufacturing, lots of efforts have been made to engineer cyanobacterial cell properties required by robust & continual cultivation and convenient & efficient recovery.
A. fimbriata has the physical features for large-scale greenhouse cultivation, including robust container growth, continuous flowering, and self-compatibility, permitting the production of large numbers of homozygous individuals required for gene functional analysis in a single life cycle.
This strain demonstrates favourable characteristics for cultivation including robust photoautotrophic growth, high neutral lipid contents containing an interesting profile of fatty acids during nitrogen starvation, pH tolerance, as well as a large cell phenotype at higher salt concentrations.
Our process resulted in the cultivation of two robust field strains that demonstrated tolerance to repetitive dosing with Omega without apparent trade-offs in productivity.
The species M. neglectum was identified in a previous screen for oleaginous microalgal strains that exhibited robust growth characteristics at varying cultivation conditions [ 19].
Transient production of recombinant scFv-Fc antibodies in HEK293-6E in combination with optimized vectors and fed batch shake flasks cultivation is efficient and robust, and integrates well into a high-throughput recombinant antibody generation pipeline.
After 10 days of cultivation, spontaneous mutants that exhibited robust growth on glycerol were isolated.
After 14 days of cultivation, evolved strains that exhibited robust growth on the inhibitor-containing medium were isolated.
Together with the observed tolerance against high pH ranges, it is feasible to suggest that M. neglectum has robust growth characteristics likely suitable for outdoor cultivation.
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