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It appears that a simple, but robust, chain topology and structural symmetry lead to high designability.
This new fusee uses a snail cam that unwinds as evenly as the spindle but allows for a thicker, more robust chain and smoother motion.
As expected, full-length HOIP alone was not active in forming ubiquitin chains, but in the presence of HOIL-1L robust chain formation was observed.
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Third, silencing pC1 neurons in male flies almost completely abolishes song-induced chaining, while activation induces robust chaining in the absence of song.
Behaviorally, silencing pC1 neurons reduced male-chaining behavior in response to pulse song, while pC1 activation is sufficient to induce robust chaining responses.
As we expected, males with feminized GRNs showed a robust chaining behavior that was indistinguishable from males with the feminization of ppk23-expressing GRNs only (supplementary material Fig. S1A).
We have also shown that song-induced male-chaining behavior is most responsive to a 35-ms IPI, although longer IPIs (35 65 ms) are still able to induce robust chaining behavior.
In contrast, CsChrimson-mediated activation of putative third-order vPN1 neurons induced robust chaining behavior, which was not observed in the absence of red light stimulation or in control flies lacking GAL4.
While activation of vPN1 induced chaining in an intensity-dependent or frequency-dependent manner, activation of pC1 generated a saturating response in which even the lowest intensity (0.006 mW/mm) or the lowest frequency (10 Hz) was able to induce robust chaining through >90% of the testing period.
The strategic design of a robust supply chain has to determine the configuration of the supply chain so that its performance remains of a consistently high quality for all possible future conditions.
Together, they have allowed Ford to work with its suppliers on mitigation strategies and create a more robust supply chain.
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