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Bacteria have remarkably robust cell shape control mechanisms.
Using this technique to create a robust cell line is tricky and highly inefficient.
"We believed we needed a more robust cell type if you go for cell-based therapies," he said.
CRISPR/Cas9-based gene targeting using synthetic guide RNAs enables robust cell biological analyses.
Infection of primary human macrophages with Salmonella elicits robust cell death and IL-1β secretion that is dependent on flagellin.
Similarly, brentuximab vedotin induced robust cell death of undifferentiated Ff-I01 (Fig. 2b), 253G1 (Fig. 2c), and 201B7 (Fig. 2d) cells.
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The few individuals who spontaneously resolve infection have robust cell-mediated immune responses.
In addition, they induce robust cell-mediated immune responses such as cytotoxic and memory T lymphocytes.
Together, our work identifies an evolvable design logic that enables robust cell-level regulation of tissue-level development.
Altogether, our findings highlight a unique microRNA-regulated mechanism that converts noisy input into a toggle switch for robust cell-fate decisions in CCSCs.
Generation of robust cell-mediated immune responses at mucosal surfaces while reducing overall inflammation is a primary goal for vaccination.
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