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Here we report a robust affinity engineering strategy using a custom designed library.
Mr. Green plays a scientist in sexual thrall to the evil mayor and displays a robust affinity for camp as both a backup singer in a girl-group love song and a tetchy hairdresser.
Finally, the Fc domain is a convenient, robust affinity handle for purification and immunochemical applications, eliminating the need for proteolytically sensitive epitope and/or affinity tags on the scFv.
The search for economical and robust affinity ligands and the synthesis of biomimetic ligands has required that chemists use molecular modeling/docking techniques.
Isolate LHI05 groups with high support within the acanthamoebids with specific and robust affinity to Protacanthamoeba bohemica (Figure 4B).
ITC measurements with the C-terminal peptide AGGPTIEEVD of Hsp70 (HSP-1 in C. elegans) revealed a weaker but robust affinity toward UNC-45 (KD ∼120 μM).
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The Internet offers the unique opportunity to organize across geography, allowing for the creation of robust global affinity groups.
To harness the robust binding affinity and selectivity of structured peptides for interactome discovery, we engineered photoreactive stapled BH3 peptide helices that covalently capture their physiologic BCL-2 family targets.
Based on the special tertiary structures, aptamers have robust binding affinity and high specificity with targets, including small organic molecules, proteins, and even cells [18 20].
Our conclusions, however, are robust across affinity groups as each group includes two or more species.
Aptamers are single-stranded DNA or RNA oligonucleotides that bind to target molecules with robust binding affinities.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com