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Here we show that phagocytosis can be a route for double-stranded RNA uptake.
Identification of receptors or transporters for dsRNAs in E. histolytica would provide a new example of selective RNA uptake by cells, allowing direct and targeted gene expression modification by exogenous genetic material, as it was initially shown in Caenorhabditis elegans [27].
We expect that ongoing SIDT1 research will identify substrate specificities and its contribution to general RNA uptake or cell contact-independent transfer of RNA.
For quantification of exosome RNA uptake, exosome-treated THP-1 cells were washed 3× with PBS to remove non-internalized exosomes.
Finally, although the weight of the current evidence may convince many that plant RNA uptake by vertebrate animals is an infrequent event, a consortium approach may be advisable to answer the question definitively à la the XMRV Scientific Research Working Group [ 114].
The list of organisms displaying environmental RNA uptake has lengthened considerably to encompass additional nematodes [ 5, 60, 61], insects [ 62– 65], even shrimp [ 66], and two species of sponge [ 67]. dsRNA can act as a species-specific pesticide in insects [ 64, 68– 71].
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The SID-1 protein from Caenorhabditis elegans was characterized as a transmembrane protein that facilitates double-stranded RNA (dsRNA) uptake and thus plays a key role in the systematic RNAi response [ 12, 13].
Given the instability of naked RNA, transcytosis – vesicular uptake of RNA carriers and their cargo (Fig. 3A) on one side and release on the other side of a biological barrier – is an attractive option (Fig. 3B).
Twenty-one studies [ 16- 18, 20- 37, 38, 38, 40] yielded 22 datasets with information about HCV RNA-positive treatment uptake levels (Table 3).
The HCV RNA-positive treatment uptake level reflects the number of study participants who received treatment as a proportion of the number of study participants known to be hepatitis C RNA-positive.
OM: outer membrane, Hb: haemoglobin, sRNA: small non-coding RNA, Fur: ferric-uptake regulator protein, COGs: Clusters of Orthologous Groups, qPCR: Real-time quantitative RT-PCR, FNR: Fumerate and Nitrate reduction Regulator, ArcA: Anoxic redox control protein A. All authors participated in conceiving and designing the study.
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