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We also thank Ms. Joan Dragavon and Dr. Jane Kuypers for coordinating HIV-1 plasma viral RNA testing for the clinical trial.
Recently, more disease-specific triage options have been emerging, such as HPV16/18 genotyping, RNA testing for a limited number of hr HPV types or p16.
Viral RNA was detected in 4 of 5 rodents that were seropositive for M or S segments; material was not available for viral RNA testing for 1 O. nasutus mouse.
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Still, sexual transmissions from survivors deemed clear of the virus have already been documented, and the World Health Organization has urged men who have recovered from Ebola to use condoms for at least 12 months after recovering from the disease or until they have two negative RNA tests for virus in their semen.
The intestinal contents were collected for RNA testing using RT-PCR, and intestinal segments were excised and immediately immersed in the different fixatives for histological examination.
We obtained the first positive ABV RNA test results for the sentinel bird 76 dpi.
The time of seroconversion was estimated based on the laboratory results of HIV-1 RNA test, ELISA test for HIV antibodies, and Western blot test as described previously [14], [15].
After ribosomal RNA removal, the RNA was tested for its ability to activate the IFN-β promoter luciferase reporter.
All 23 predicted regions, except the ones of the GCVT riboswitch, the FMN riboswitch and the 6S RNA, were tested for transcription of small-size RNA by Northern blot analyses.
RNA was tested for degradation and purity using an Agilent 2100 Bioanalyzer and RNA 6000 Nano kit (Agilent Technologies).
The resulting RNA was tested for quality with an Agilent 2100 Bioanalyzer, converted to labeled complementary RNA (cRNA) and hybridized to Drosophila 2 3′-UTR microAffymetrixfymetrix).
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