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The RNA labelling could therefore correspond to an RNA template dependent RNA synthesis.
Additionally, weight 159.0 gm/mole is added that accounts to 5'triphospahte: (2) Free energy δ G : δ G = RT ln RNA · template RNA template.
The rice U6 RNA was used as the internal control for RNA template normalization.
The RNA template used for qPCR was the same RNA that was used for microarray hybridizations.
They first transcribe a positive RNA template, then use that template to regenerate their negative RNA genome.
Oligonucleotides for preparing RNA template.
The cell then makes the protein from this RNA template — the messenger.
The TERC gene encodes the RNA template of the telomerase.
Phen-DC3 was mixed with the RNA template before adding HCV RdRp, then the level of RNA synthesis was measured (Fig. 3A).
To prevent hair-pinning, the first strand was reverse-transcribed off the RNA template in the presence of Actinomycin D, followed by SPRI bead purification.
Telomerase uses a unique mode of primer extension, whereby an internal RNA template is used to specify the sequence of the added repeat10.
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