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RNA samples exhibiting an RNA integrity number (RIN) greater than 6.5 were included in the study.
All extracted RNA samples were analyzed for RNA integrity by Mayo Clinic's Advanced Genomic Technology Center Gene Expression Core to exclude any RNA samples unsuitable for analysis.
The cells were collected for RNA samples.
Kim prepared DNA and RNA samples.
Total RNA samples were prepared using TRIzol (Thermo Fisher Scientific).
prepared DNA and RNA samples and maintained sample archives.
B.C.M. prepared the DNA samples and RNA samples.
For eliminating genomic DNA, RNA samples were treated with DNase I (6 u/ul) (Zymo Research).
In brief, the quality of RNA samples was examined by Agilent bioanalyzer (Agilent).
After transcription, RNA samples were heat denatured and purified by using urea-denaturing polyacrylamide gels.
The adaptor-ligated RNA samples were purified using 2.4-fold excess AMPure XP beads (Agencourt).
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