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RiboRuler High Range RNA Ladder (Thermo Scientific) ranging from 200 to 6000 nt was used as RNA marker.
The Decade™ Marker System kit (Ambion) was used to prepare radiolabeled RNA marker with radioactive [γ-32P]-dATP.
Finally, a ribosomal RNA marker for the 28S rRNA gene optimised by Naito et al. [J. Forensic Sci. 37 (1992) 396] was used as a possible marker for speciation.
While some transcripts are fetal-specific in maternal whole blood, e.g. CSHL1, due care is needed to validate each new potential whole blood transcript that is meant to be used as a circulating fetal RNA marker.
The RNA marker ladder was generated by alkaline hydrolysis as described previously [ 16].
A RNA marker was run in parallel as a molecular-sized standard.
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RNA markers are indicated on the right margin.
The purpose of this study was to determine whether the combined distribution of a panel of cellular messenger RNA markers can detect preeclampsia long before onset.
The purpose of this study was to predict the occurrence of preeclampsia in a series of patients at gestational week 15-20 weeks, with the use of a panel of messenger RNA markers.
By using a gene chip to compare the salivary RNA of people in each disease group to that of 10 healthy people, the researchers found candidate RNA markers linked to Sjogren's syndrome (26 RNAs), type II diabetes (126 RNAs), and breast cancer (103 RNAs).
RNA markers with 23 and 21 nt were 5'-end labeled by the same method.
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