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A 6×-histidine tag was fused to the N-terminus to facilitate purification of the recombinant LAP (rLAP) by immobilized-metal ion-affinity chromatography (IMAC).
Thus, this work provides an rLAP that can be used in industrial processes for N-terminal methionine excision (NME) from such recombinant products as interferon α-2b, human growth hormone and granulocyte macrophage-colony stimulating factor.
In addition, a kinetic characterization of rLAP was performed.
Therefore, rLAP can be used as a biologically active therapeutic protein.
Recombinant latency associated protein (rLAP, dimer of the pro-region non-covalently associated with the mature region) complex and mature TGF-beta 1 induce apoptosis in isolated hepatocytes cultured in vitro.
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Based on [18, Theorem 2], ({mathbf {rlap h}}_{RD}^{H}{left ({{{mathbf {T}}^ bot }} right)^{H}}{{mathbf {T}}^ bot }{{mathbf {rlap h}}_{RD}}) is a chi-square random variable with 2(M−1) degrees of freedom.
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