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PCR conditions on Rotor-Gene Q (Qiagen Hilden, Germany) were as follows: 95°C for 5 minutes, then 40 cycles of 95°C for 30 seconds, 56.6°C for 30 seconds and 72°C for 30 seconds, followed by a melting profile with a ramping range of 65° to 95°C and rising steps of 1 degree.
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Suddenly, he rose, stepped out of his shop and shuttered it.
While the bass sings the line first, the tenor adds a sequence of sustained notes rising step by step one fifth.
Occurrence of condom accidents rose step-wise with each increase in AUDIT category.
They converged, one by one, on a nondescript Manhattan high-rise, stepping out of sport utility vehicles and hurrying in with briefcases: a quiet gathering of New York City government's highest-ranking officials.
Last September the city unveiled plans for a 625-foot-tall Ferris wheel, an outlet mall, and a 200-room hotel to rise within steps of the ferry terminal.
But steam was rising from the steps of city hall, three blocks away, where Hillary Clinton was venting her rage at the Bush administration for having lied to the American people.
Because the distribution was rising in steps and the assumptions of continuity did not hold, we categorized the costs of social care (MODEL 3).
Many who protested today blame corporations for putting capital before environmental protection, and intended to rise to the steps of the New York Stock Exchange and stage a massive sit-in.
Second, the two-photon imaging experiments indicated the occurrence of large exocytotic events that rose without discernible steps of the type seen during sequential exocytosis (compare Fig 5c and g).
The journalist, Toni Locy, was ordered last week by a federal judge, Reggie B. Walton, to pay a daily fine of $500, rising in steps to $5,000, for refusing to name the sources for an article she wrote about Steven J. Hatfill, a former Army bioweapons scientist named as a "person of interest" in the 2001 anthrax attacks.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com