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To analyse the functions of genomic loci involved by RIS, we collected data regarding the gene directly hit or the single gene closest to each insertion site in order to assign one gene to one integration.
Under RIS, we predicted that the insular lizard (1) develops faster in order to mature early, (2) is more sexually dimorphic as a consequence of strong sexual selection, and (3) has slower growth rate as a consequence of re-allocation of resources from growth into reproduction [ 21].
Using the same 24 RIS, we extracted total RNA from cardiac left ventricles from four male mice for each strain and used Illumina MouseRef-8 v2.0 BeadChips to obtain the profile of gene expression in the tissues, as described previously (Llamas et al. 2009).
Though in our study we could not demonstrate that previous radiotherapy was an independent prognosticator in RIS, we cannot exclude that radiotherapy has led to the demonstrated clustering of unfavourable prognostic factors as evident for incomplete surgery and central tumour site.
This result suggests that the RIs we found for the real heterotypic pairs reflect the degree of co-regularity to be expected between two independent, but highly ordered mosaics [14].
In this same corpus of papers, in addition to the OxyR RIs, we identified 29 RIs for a variety of TFs, with their corresponding GCs.
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Change in voracity (food intake rate) is not directly predicted by RIS, but we expected it to occur if increased MCR expression triggers RIS, as we hypothesize.
Finally, to test whether regions containing cis-eQTL clusters in one tissue would overlap with regions containing cis-eQTL clusters in one same tissue from two RIS panels, we compared data obtained for eye genes from AxB/BxA and BxD RIS.
Since these coordinated traits partly overlap with the trait-shifts we predicted to occur under RIS (Table 1), we predicted that increased MCR expression levels could be the proximal, physiological causes of the RIS.
In addition, we evaluated Ris effects on osteocyte cells in vitro, and we observed similar results obtained on osteoblasts.
RI Period and learning period length: Following R&C, we explore RIs with both R = 12 and R = 36 months.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com