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SlAP2a is induced during ripening, however, silencing using RNAi caused higher levels of ethylene and carotenoids culminating in advanced ripening status.
These fruits were then pooled according to ripening status for analysis.
In order to reach a more precise idea of the ripening status, it may also be useful to combine a ratio or difference of activities of both up- and down-regulated genes/proteins/metabolites rather than to rely only on up- or down-regulated genes.
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In addition, the candidate genes could be examined as molecular markers to indicate the status of ripening capacity, as well as determining appropriate postharvest treatments.
The information provided by thermal images proved to be relevant at a seasonal scale as well, although it did not match seasonal trends in water status but mimicked other physiological processes occurring during ripening.
The score scatter plot indicates that the main sources of variance were related to the ripening stages (PC1) and the cultivars (PC2) rather than the transgenic status of the plants.
However, the dominant transposon-tagged tomato mutant Orr, which has a defect in the NDH (NADH dehydrogenase) complex leading to an altered plastid redox status, has reduced carotenoid accumulation in the chromoplasts during fruit ripening.
Hence, during ripening glycolysis would be controlled strictly by accumulated PEP and by the energy status of mesocarp cells.
Proteomic analysis of tomato during ripening in two ecotypes revealed 83 proteins that were differentially expressed involved with redox status, defense, stress, carbon metabolism, energy production and cellular signaling.
Ripening grapefruit.
This inhibits ripening.
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