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The period between 8 and 10 wpf, named véraison, represents the transition to ripening during which crucial events occur: dramatic opposing changes of organic acids and sugars contents in the pulp, softening of the fruit and coloring of the skin.
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Finally, when the supersaturation was depleted, Ostwald ripening occurred, during which large particles grew by means of sacrificing small ones.
CaACO4, which may be considered to be a System 1-associated isoform, was up-regulated during ripening onset which suggests that its up-regulation might be closely associated with these ripening regulators in an ethylene-independent manner.
The remaining 20% of genes identified only by the direct comparison at each time point represent genes with a less than five-fold difference in expression between C and CT during ripening, and which were therefore excluded from the first cluster analysis.
Fruit hypertrophy begins the following spring with ripening during summer.
Accordingly, the levels of this compound decreased during the ripening of the 'Granada' genotype and after the shelf-life ripening in 'MxR_01', which is in accordance with the shift of aroma from immature to ripe fruit.
The members of the expansin and xyloglucan transglycosylase/hydrolase (XTH) gene families were highly up-regulated during ripening, which suggests that they might play important roles in the softening of the fruit.
A study by Lara et al. followed the recovery of the fruit from the heat stress, and the resumption of ripening which was inhibited during HAT, particularly softening and ethylene gene expression.
Even Alain Vircondelet, the most credulous of her three biographers, calls the story a legend she invented, which, "having ripened during her whole life, finally became true".
These traits are shaped during the complex process of ripening, which although vary among species, is yet associated with typical cellular activity [ 1- 4].
Three isoenzymes of an isoflavone reductase-like protein (IFRL) have been reported as being up-regulated during ripening in the mesocarp, which confirms previous results obtained in the grape berry mesocarp and skin [ 31, 36].
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